{"id":3340,"date":"2019-08-27T10:15:04","date_gmt":"2019-08-27T01:15:04","guid":{"rendered":"http:\/\/hiha.hiroshima-u.ac.jp\/?p=3340"},"modified":"2019-10-24T09:34:11","modified_gmt":"2019-10-24T00:34:11","slug":"19th-august-a-new-research-paper-based-upon-international-collaboration-between-takashi-todas-group-and-the-francis-crick-institute-london-uk-was-published-in-the-journal-of-cell-science-o","status":"publish","type":"post","link":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/2019\/08\/27\/19th-august-a-new-research-paper-based-upon-international-collaboration-between-takashi-todas-group-and-the-francis-crick-institute-london-uk-was-published-in-the-journal-of-cell-science-o\/","title":{"rendered":"19th August A new research paper based upon International collaboration between Takashi Toda\u2019s group and the Francis Crick Institute (London, UK) was published in the Journal of Cell Science online."},"content":{"rendered":"
19th August<\/span><\/div>\r\n
A new research paper based upon International collaboration between Takashi Toda\u2019s group and the Francis Crick Institute (London, UK) was published in the Journal of Cell Science online. This work is partly supported by\u00a0\u201cProgram For Fostering Globally Talented Research\u201d \u00a0JSPS grant (S2902\/JPMX05S2900002). The first\u00a0author Dr Corinne Pinder stayed in\u00a0Takashi Toda\u2019s laboratory in 2018 (January-May) to carry out this\u00a0research project.\u00a0<\/span><\/div>\r\n
\u00a0<\/span><\/div>\r\n
Authors<\/span><\/div>\r\n
Corinne L. Pinder, Yuzy Matsuo, Sebastian P. Maurer\u00a0<\/span>and Takashi\u00a0Toda<\/span><\/span><\/div>\r\n
\u00a0<\/span><\/div>\r\n
The title<\/span><\/div>\r\n
Kinesin-8 and TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation<\/span><\/div>\r\n
\u00a0<\/span><\/div>\r\n
Abstract<\/span><\/span><\/div>\r\n
High-fidelity chromosome segregation relies on proper microtubule regulation. Kinesin-8 has been shown to destabilise microtubules to reduce metaphase spindle length and chromosome movements in multiple species. XMAP215\/chTOG polymerases catalyse microtubule growth for spindle assembly, elongation and kinetochore-microtubule attachment. Understanding of their biochemical activity has advanced but little work directly addresses the functionality and interplay of these conserved factors. We utilised the synthetic lethality of fission yeast kinesin-8 (Klp5-Klp6) and XMAP215\/chTOG (Dis1) to study their individual and overlapping roles. We found that the non-motor kinesin-8 tailbox is essential for mitotic function; mutation compromises plus-end-directed processivity. Klp5-Klp6 induces catastrophes to control microtubule length and su<\/span>r<\/span>prisingly, Dis1 collaborates with kinesin-8 to slow spindle elongation. Together, they enforce a maximum spindle length for a viable metaphase-anaphase transition and limit elongation during anaphase A to prevent lagging chromatids. Our work provides mechanistic insight into how kinesin-8 negatively regulates microtubules and how this functionally overlaps with Dis1 and highlights the importance of spindle length control in mitosis.<\/span><\/span><\/div>\r\n
\u00a0<\/span><\/span><\/div>\r\n
URL<\/span><\/span><\/div>\r\n
https:\/\/jcs.biologists.org\/content\/early\/2019\/08\/17\/jcs.232306<\/span><\/a><\/div>\r\n
\u00a0<\/span><\/span><\/div>\r\n
Journal<\/span><\/span><\/div>\r\n
Journal of Cell Science\u00a0<\/span>2019\u00a0<\/span>:\u00a0<\/span>jcs.232306\u00a0<\/span>doi:\u00a010.1242\/jcs.232306<\/span><\/span><\/div>","protected":false},"excerpt":{"rendered":"19th August A new research paper based upon International collaboration between Takashi Toda\u2019s group and the Francis Crick Institute (London, UK) was published in the Journal of Cell Science online. This work is partly supported by\u00a0\u201cProgram For Fostering Globally Talented Research\u201d \u00a0JSPS grant (S2902\/JPMX05S2900002). The first\u00a0author Dr Corinne Pinder stayed in\u00a0Takashi Toda\u2019s laboratory in 2018 (January-May) to carry out this\u00a0research project.\u00a0 \u00a0 Authors Corinne L. Pinder, Yuzy Matsuo, Sebastian P. Maurer\u00a0and Takashi\u00a0Toda \u00a0 The title Kinesin-8 and TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation \u00a0 Abstract High-fidelity chromosome segregation relies on proper microtubule regulation. Kinesin-8 has been shown to destabilise microtubules to […]","protected":false},"author":5,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[6,7,1],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/posts\/3340"}],"collection":[{"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/comments?post=3340"}],"version-history":[{"count":0,"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/posts\/3340\/revisions"}],"wp:attachment":[{"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/media?parent=3340"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/categories?post=3340"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/wp-json\/wp\/v2\/tags?post=3340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}