{"id":2736,"date":"2019-01-15T09:00:52","date_gmt":"2019-01-15T00:00:52","guid":{"rendered":"http:\/\/hiha.hiroshima-u.ac.jp\/?p=2736"},"modified":"2019-01-15T09:17:55","modified_gmt":"2019-01-15T00:17:55","slug":"a-new-paper-from-drs-takashi-toda-and-masashi-yukawa-group-was-accepted-in-g3-and-published-online","status":"publish","type":"post","link":"https:\/\/hiha.hiroshima-u.ac.jp\/en\/2019\/01\/15\/a-new-paper-from-drs-takashi-toda-and-masashi-yukawa-group-was-accepted-in-g3-and-published-online\/","title":{"rendered":"A new paper from Drs Toda and Yukawa group was published in G3: Genes, Genomes, Genetics."},"content":{"rendered":"
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\uff19th January<\/span><\/div>\r\n
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A new paper from Drs Toda and Yukawa group was published in G3: Genes, Genomes, Genetics.<\/span><\/div>\r\n
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January 1, 2019\u00a0<\/span>vol. 9\u00a0<\/span>no. 1\u00a0<\/span>269-280;\u00a0<\/span>https:\/\/doi.org\/10.1534\/g3.118.200896<\/a><\/span><\/div>\r\n\r\n\u3010G3<\/a>\u3011\u3010PubMed<\/a>\u3011<\/div>\r\n
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Title\uff1aSuppressor analysis uncovers that MAPs and microtubule dynamics balance with the Cut7\/Kinesin-5 motor for mitotic spindle assembly in\u00a0Schizosaccharomyces pombe<\/i><\/div>\r\n
Authors\uff1aMasashi Yukawa, Yusuke Yamada\u00a0and Takashi Toda<\/div>\r\n
Journal\uff1aG\uff13<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n
Abstract:\u00a0The Kinesin-5 motor\u00a0<\/i>Cut7 in\u00a0Schizosaccharomyces pombe<\/i>\u00a0plays essential roles in spindle pole separation, leading to the assembly of bipolar spindle. In many organisms, simultaneous inactivation of Kinesin-14s neutralizes Kinesin-5 deficiency. To uncover the molecular network that counteracts Kinesin-5, we have conducted a genetic screening for suppressors that rescue the\u00a0cut7-22<\/i>\u00a0temperature sensitive mutation, and identified 10 loci. Next generation sequencing analysis reveals that causative mutations are mapped in genes encoding \u03b1-, \u03b2-tubulins and the microtubule plus-end tracking protein Mal3\/EB1, in addition to the components of the Pkl1\/Kinesin-14 complex. Moreover, the deletion of various genes required for microtubule nucleation\/polymerization also suppresses the\u00a0cut7<\/i>\u00a0mutant. Intriguingly, Klp2\/Kinesin-14 levels on the spindles are significantly increased in\u00a0cut7<\/i>\u00a0mutants, whereas these increases are negated by suppressors, which may explain the suppression by these mutations\/deletions. Consistent with this notion, mild overproduction of Klp2 in these double mutant cells confers temperature sensitivity. Surprisingly, treatment with a microtubule-destabilizing drug not only suppresses\u00a0cut7\u00a0<\/i>temperature sensitivity\u00a0<\/i>but also rescues the lethality resulting from the deletion\u00a0<\/i>of\u00a0cut7<\/i>, though a single\u00a0klp2<\/i>\u00a0deletion per se cannot compensate for the loss of Cut7. We propose that microtubule assembly and\/or dynamics antagonize Cut7 functions, and that the orchestration between these two factors is crucial for bipolar spindle assembly.<\/div>\r\n
\u00a0<\/div>\r\n<\/div>","protected":false},"excerpt":{"rendered":"\uff19th January \u00a0 A new paper from Drs Toda and Yukawa group was published in G3: Genes, Genomes, Genetics. \u00a0 January 1, 2019\u00a0vol. 9\u00a0no. 1\u00a0269-280;\u00a0https:\/\/doi.org\/10.1534\/g3.118.200896 \u3010G3\u3011\u3010PubMed\u3011 \u00a0 Title\uff1aSuppressor analysis uncovers that MAPs and microtubule dynamics balance with the Cut7\/Kinesin-5 motor for mitotic spindle assembly in\u00a0Schizosaccharomyces pombe Authors\uff1aMasashi Yukawa, Yusuke Yamada\u00a0and Takashi Toda Journal\uff1aG\uff13 Abstract:\u00a0The Kinesin-5 motor\u00a0Cut7 in\u00a0Schizosaccharomyces pombe\u00a0plays essential roles in spindle pole separation, leading to the assembly of bipolar spindle. In many organisms, simultaneous inactivation of Kinesin-14s neutralizes Kinesin-5 deficiency. To uncover the molecular network that counteracts Kinesin-5, we have conducted a genetic screening for suppressors that rescue the\u00a0cut7-22\u00a0temperature sensitive mutation, and identified 10 loci. Next generation sequencing analysis […]","protected":false},"author":5,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[6,7,1],"tags":[],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t